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Old July 11, 2013   #16
ChrisK
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You might find this paper interesting. Think I have posted it before.
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Old July 15, 2013   #17
Minnesota Mato
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I am also looking to breed for improved beta- carotene. Maybe we could share info. Here is my latest, a F1 cross between a galapagos tomato and a pink heart. The plant is huge and the tomatoes are bigger then normal for a cross with a wild galapagos. Now I just have to select for orange colored fruit and increased size.
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Old July 15, 2013   #18
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Quote:
Originally Posted by Minnesota Mato View Post
I am also looking to breed for improved beta- carotene. Maybe we could share info. Here is my latest, a F1 cross between a galapagos tomato and a pink heart. The plant is huge and the tomatoes are bigger then normal for a cross with a wild galapagos. Now I just have to select for orange colored fruit and increased size.
I'd be glad to share information (and maybe even breeding stock), but I won't get my F1 cross until next year, as I didn't have the tomatoes (of the right kind) or my plan worked out until this summer. So I using this summer as a 'practice' for tomato breeding [I'd estimate that only about 1/3 of my crosses are 'taking'. Is this fairly standard for a beginner at tomato crossing?.] I'm not familiar with the Galapagos tomato. What's it like?

What I learned so far (plus questins):
1) Not all orange colors are created equal
2) Elevated beta carotene results from the presence of the gene B (The
tomato is orange-red) the highest levels result from the additional precense
of the modifier gene MOG (and an orange color).
3) Question: will high levels of beta-carotene cause unfavorable flavors? Can
this be bred around?
4) B is on the same chromosome as the gene for determinate growth and is
linked to it. Can this link be broken?

Next year I plan that my main tomato breeding will involve CaroRich, 97L97, and Opalka.
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Old July 15, 2013   #19
joseph
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I prefer the taste of high carotene foods to low carotene versions of the same food.

Genes crossover between the paired chromosomes, so it's possible to break the linkage between two genes on the same chromosome. How frequently that happens depends on how close together the genes are located on the chromosome. If they are on opposite ends of the chromosome it's like they are not linked at all. If they are side by side then it would be much less likely to break the linkage. If you can find a gene-map containing both genes, and it has a centimorgan scale on it, then that is a measures the likelihood of the linkage being broken in a cross. 1 centimorgan = 1% chance. So if they are tightly linked you just have to grow more plants to break the linkage.

Here's an example of a gene map. In this particular cross, chromosomes 3 and 8 contain a QTL for carotene content. The B gene is located on Chromosome 6. So there are lots of opportunities for increased carotene content.

Last edited by joseph; July 15, 2013 at 01:58 PM.
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Old July 16, 2013   #20
Minnesota Mato
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I would like to trade info and breeding stock. To be more precise the tomatoes come from the Galapagos islands but are called CHEESMANII. There are alot of cool things about cheesmanii but crossing with them is like starting from the beginning. Crossing to get the B gene is easy but keeping all the positives and getting a decent size is hard. You should google cheesmanii.
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Old July 26, 2013   #21
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Quote:
Originally Posted by Minnesota Mato View Post
I am also looking to breed for improved beta- carotene. Maybe we could share info. Here is my latest, a F1 cross between a galapagos tomato and a pink heart.
Has your cross started to ripen yet? How does it seem so far?
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Old July 27, 2013   #22
Minnesota Mato
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I am already fermenting the seeds! I am going to try to plant a few and see what I can do before winter. Both of my crosses so far have been a nice deep orange. I need to start selecting for greater size now.
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Old August 16, 2017   #23
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Default Odd result in the F2

I can't post pictures at this time but I'm having an 'odd' result in the F2. I crossed Opalka X 97L97. Opalka is a large pointed heirloom indeterminate paste type and 97L97 is a determinate paste colored orange because it carries the B gene.

The F1 seemed to be as expected, there was slight size differences in fruit size and shape between the plants, but all the plants were indeterminate, orange colored, and about the size of 97L97.

I expected significant variation in the F2. I got them in late after significant groundhog damage, so none have ripened yet. I expected size variation, and some shape variation but all 7 of my F2s appear to be small, nippled ,longer than wide fruit that are much smaller than either original parent. I guess I have a problem with cross pollination, but is there any other thoughts why I would be seeing this? I would think I would see more variation given the F1 parent was a hybrid. The fruits thus far seem almost identical like I was at an F5 or something.

Please post any ideas you may have.
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Old August 26, 2017   #24
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Chris, I had similar unexpected results - where the F2 were all very very similar to my F1 and looked to be easy to stablilize that type, but then the F3 turned out to be a very mixed bag.
Another one was at F4 and seemed to be quite stable, but then the F5 suddenly saw a load of segregation for shape. These were hearts that segregated into many subtypes including paste shapes, nipples or not, etc.

My impression is that the shape genetics of pointy fruit are very very complex. There may be some epistasis involved which prevents things being expressed as expected. Then with another shuffle, bingo out they come. Pretty baffling.
I was reading about it this spring, there are ??? seven I think different genes for 'pointy'. So crosses of two pointy fruit won't necessarily segregate as expected if there were only one gene for that.
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Old August 26, 2017   #25
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Thanks bower - that at least helps make some sense.

I believe I am going to revise my approach. I think I am going to do some (F1XF1) crosses and (F1XHibeta). The F1s are already (XHibeta).

Quote:
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Chris, I had similar unexpected results - where the F2 were all very very similar to my F1 and looked to be easy to stablilize that type, but then the F3 turned out to be a very mixed bag.
Another one was at F4 and seemed to be quite stable, but then the F5 suddenly saw a load of segregation for shape. These were hearts that segregated into many subtypes including paste shapes, nipples or not, etc.

My impression is that the shape genetics of pointy fruit are very very complex. There may be some epistasis involved which prevents things being expressed as expected. Then with another shuffle, bingo out they come. Pretty baffling.
I was reading about it this spring, there are ??? seven I think different genes for 'pointy'. So crosses of two pointy fruit won't necessarily segregate as expected if there were only one gene for that.
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Old August 28, 2017   #26
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Default Changing Approach

Well my F2's have mostly come in and their consistency is amazing (I earlier described them as 'nippled', but when fully developed they are an oval.

All the fruit are all ovals and about 1-1/2 inches long by about 1 inch. The are all orange with the skins blushed with a red hue. There is slight internal wall thickness variation, but is slight enough that I decided not to differentiate on that.

So all my F3 that have produced tomatoes so far (Plants 1 through 5), I decided not to keep separately, but am fermenting them together.

The Jaune Flamme X Shannon (plant 2) F2, though it was the shape and size I wanted, and appears very productive, was red, was very juicy, and the fruit walls were thin.

I might grow some out in 2018 to see if I get any differentiation, but I am planning several different approaches now.

One of the problems I'm fight is the following 97L97 has high Beta gene with the modifier gene (for higher beta carotene, but is determinate (but is a paste type)

Jaune Flamme has the high Beta gene and is indeterminate, but does not have the modifier gene (and is not a paste type).

The high Beta gene is on the same chromosome as the sp gene (Determinate) so is 'linked' to it (and is close enough that breakage between the two is fairly rare.

I'm looking to develop a paste that is indeterminate, and has both the high Beta and the Modiere


First, I'm planning (Jaune Flamee X Shannon) X (97L97 X Opalka) (and vice versa)

Second Backcross to High Beta (Jaune Flammee X Shannon) X 97L97
AND (97L97 X Opalka) X Jaune Flammee.

Any thoughts?
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Old August 29, 2017   #27
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Thinking about the beta linkage... Last year I grew six F3 plants of a heart or paste shaped selection - from an F2 that was Beta/- (orange-red) and indeterminate. I expected to get 1/4 (roughly!) that was Beta/Beta and determinate because of the linkage (Zolotoe Serdtse parent line), but instead I had more Beta/- indeterminates again and no determinates at all in the six.

You made that great post about odds awhile ago, do you think you could up the ante just by growing more plants from your F2 or F3? I thought the result was 'unlucky' for me but it's not out of the question with half dozen plants.

I also like your strategy of crossing unstable generations, and of crossing sp/Beta with indeterminate Beta, where both conditions are linked, what will happen? Would be nice to see the result of some larger numbers in that cross.
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Old August 31, 2017   #28
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Quote:
Originally Posted by bower View Post
Thinking about the beta linkage... Last year I grew six F3 plants of a heart or paste shaped selection - from an F2 that was Beta/- (orange-red) and indeterminate. I expected to get 1/4 (roughly!) that was Beta/Beta and determinate because of the linkage (Zolotoe Serdtse parent line), but instead I had more Beta/- indeterminates again and no determinates at all in the six.

You made that great post about odds awhile ago, do you think you could up the ante just by growing more plants from your F2 or F3? I thought the result was 'unlucky' for me but it's not out of the question with half dozen plants.
1) You've given me an interesting idea (more later) Thanks!
2) I started playing with some Punnett squares, assuming there is no breakage (Beta and self-pruning are very close on the chromosome). I run into problems with the existing F1's crossed together, I would get 3/4 orange-red and 1/4 red, but only 1/3 of the orange-reds have the indeterminate high beta with the modifier gene present but hidden. Think I'll still do it, but I'll have to grow out a good amount of progency of the 2nd generation to find what I'm looking for.
3) This is the 'good' idea you've given me. I'm going to cross my two beta varieties, and select for indeterminate + Modifier (3/16) Only then will I cross the resulting tomato with pastes.
4) The other crosses I talked about (F1 X high beta) I may yet pursue but I'll need to analyze the potentials with more Punnett squares)


Quote:
Originally Posted by bower View Post
I also like your strategy of crossing unstable generations, and of crossing sp/Beta with indeterminate Beta, where both conditions are linked, what will happen? Would be nice to see the result of some larger numbers in that cross.
I'm going to probably do that as well (I also saved seed from the same F1 cross, but a different plant, so I can see if get a different result.
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