2013 breeding garden

[carolyn137]

Originally Posted by goodwin

It came up in something I was reading a while back on leaf morphology - a bit dense, but worth digging through if you are interested.
http://www.plantcell.org/content/23/10/3595.full
The research is fairly recent (2011)

Lee

Thanks Lee and I do get the main points but there were many genes I didn't know by name, and nowI do.

I'm surpirsed that Arabdopsis is still being used as a model system but since it's so easy to grow and study I shouldn't be surprised.

Carolyn

[ChrisK]

Nice paper. Thanks for posting it.

Originally Posted by goodwin

It came up in something I was reading a while back on leaf morphology - a bit dense, but worth digging through if you are interested.
http://www.plantcell.org/content/23/10/3595.full
The research is fairly recent (2011)

Lee

[FishMato]

The black krim seeds have already sprouted. They were planted around three days ago, along with the Cherokee purple and some cherry tomatoes. The other charities were planted late and have not come up yet. I'm clearing the are for my breeding garden tomorrow . I will be adding mushroom compost, some topsoil, black kow and some perlite.

[FishMato]

All the seeds hav came up. I am starting to clear the its where they will be grown. Since this is my first time growing heirloom tomatoes, I am doing a sort of side by side test plot to test factors such as yield, disease resitance, drought tolerance and so on as well as some breeding.

[crmauch]

Originally Posted by FishMato

All the seeds hav came up. I am starting to clear the its where they will be grown. Since this is my first time growing heirloom tomatoes, I am doing a sort of side by side test plot to test factors such as yield, disease resitance, drought tolerance and so on as well as some breeding.

How has your breeding gone?

[frogsleap farm]

I apologize if this comment is far from the original topic of this thread. I am homozygous for the geek gene. A mutation that leads to a deletion (single base pair or larger) almost always leads to a loss of function mutant - a mutant plant for which the mutant gene is non-funtional. As DarJones points out there were several independent loss of function mutants in the gene for chlorophyll degeneration in the fruit, all leading to the retained chlorophyll (or black) phenotype. They are allelic - acting as the same allele for the gf gene. Based on the paper Lee references, PL is controlled by a transcription factor (regulatory gene) that controls expression of one or more functional genes (as is the gene controlling yellow vs clear epidermis - red vs pink). Transcription factors can control multiple genes in multiple pathways, thus the "pleiotropic" effects Dar references. Aft, controlling anthocyanin production in tomato fruit, is another interesting transcription factor - now understood to also influence delayed ripening. I also agree with Dar that a reversion mutation PL to RL is very unlikely.

[goodwin]

frogsleap,
Good to hear from you, and an apt comment. What you say about Aft would explain some things.
I have your Indigo Tiger in the second year of full production and have separated two strains, but will post the results of that in the other thread.
It looks like you are doing some interesting work as usual.
Lee

[ChrisK]

Since I am also homozygous for geekness...loss of 1 or 2 bp leads to loss of function because reading frame changes. Loss of 3 consecutive bases (or multiple thereof) would not necessarily do so as reading frame is maintained, and proteins often retain function with this type of mutation. Same goes for insertion of bases.

Originally Posted by frogsleap farm

I apologize if this comment is far from the original topic of this thread. I am homozygous for the geek gene. A mutation that leads to a deletion (single base pair or larger) almost always leads to a loss of function mutant -

[frogsleap farm]

Originally Posted by ChrisK

Since I am also homozygous for geekness...loss of 1 or 2 bp leads to loss of function because reading frame changes. Loss of 3 consecutive bases (or multiple thereof) would not necessarily do so as reading frame is maintained, and proteins often retain function with this type of mutation. Same goes for insertion of bases.

Chris - thus my almost always qualifier. A frameshift mutation is a no brainer loss of function, deletion of one or more amino acids (one or more sets of 3bp deletions) may or may not, depending on whether the amino acid deletion(s) is/are in a critical region of the protein.

[ChrisK]

Correct. Just wanted to be clear (or maybe just overly pedantic)

Originally Posted by frogsleap farm

Chris - thus my almost always qualifier. A frameshift mutation is a no brainer loss of function, deletion of one or more amino acids (one or more sets of 3bp deletions) may or may not, depending on whether the amino acid deletion(s) is/are in a critical region of the protein.