Forum area for discussing hybridizing tomatoes in technical terms and information pertinent to trait/variety specific long-term (1+ years) growout projects.
|
|
Thread Tools | Display Modes |
March 5, 2013 | #16 | |
Moderator Emeritus
Join Date: Jan 2006
Location: Upstate NY, zone 4b/5a
Posts: 21,169
|
Quote:
I'm surpirsed that Arabdopsis is still being used as a model system but since it's so easy to grow and study I shouldn't be surprised. Carolyn
__________________
Carolyn |
|
March 5, 2013 | #17 | |
Tomatovillian™
Join Date: Mar 2011
Location: Raleigh, NC
Posts: 1,448
|
Nice paper. Thanks for posting it.
Quote:
__________________
Blog: chriskafer.wordpress.com Ignorance more frequently begets knowledge: it is those who know little, and not those who know much, who so positively assert that this or that problem will never be solved by science. --Charles Darwin |
|
March 7, 2013 | #18 |
Tomatovillian™
Join Date: Jan 2013
Location: Tallahassee, FL
Posts: 9
|
The black krim seeds have already sprouted. They were planted around three days ago, along with the Cherokee purple and some cherry tomatoes. The other charities were planted late and have not come up yet. I'm clearing the are for my breeding garden tomorrow . I will be adding mushroom compost, some topsoil, black kow and some perlite.
|
March 11, 2013 | #19 |
Tomatovillian™
Join Date: Jan 2013
Location: Tallahassee, FL
Posts: 9
|
All the seeds hav came up. I am starting to clear the its where they will be grown. Since this is my first time growing heirloom tomatoes, I am doing a sort of side by side test plot to test factors such as yield, disease resitance, drought tolerance and so on as well as some breeding.
|
July 25, 2013 | #20 | |
Tomatovillian™
Join Date: May 2013
Location: Honey Brook, PA Zone 6b
Posts: 399
|
Quote:
|
|
July 25, 2013 | #21 |
Tomatovillian™
Join Date: Nov 2008
Location: Minnesota
Posts: 568
|
I apologize if this comment is far from the original topic of this thread. I am homozygous for the geek gene. A mutation that leads to a deletion (single base pair or larger) almost always leads to a loss of function mutant - a mutant plant for which the mutant gene is non-funtional. As DarJones points out there were several independent loss of function mutants in the gene for chlorophyll degeneration in the fruit, all leading to the retained chlorophyll (or black) phenotype. They are allelic - acting as the same allele for the gf gene. Based on the paper Lee references, PL is controlled by a transcription factor (regulatory gene) that controls expression of one or more functional genes (as is the gene controlling yellow vs clear epidermis - red vs pink). Transcription factors can control multiple genes in multiple pathways, thus the "pleiotropic" effects Dar references. Aft, controlling anthocyanin production in tomato fruit, is another interesting transcription factor - now understood to also influence delayed ripening. I also agree with Dar that a reversion mutation PL to RL is very unlikely.
|
July 25, 2013 | #22 |
Tomatovillian™
Join Date: Dec 2007
Location: Espanola, New Mexico
Posts: 606
|
frogsleap,
Good to hear from you, and an apt comment. What you say about Aft would explain some things. I have your Indigo Tiger in the second year of full production and have separated two strains, but will post the results of that in the other thread. It looks like you are doing some interesting work as usual. Lee |
July 26, 2013 | #23 |
Tomatovillian™
Join Date: Mar 2011
Location: Raleigh, NC
Posts: 1,448
|
Since I am also homozygous for geekness...loss of 1 or 2 bp leads to loss of function because reading frame changes. Loss of 3 consecutive bases (or multiple thereof) would not necessarily do so as reading frame is maintained, and proteins often retain function with this type of mutation. Same goes for insertion of bases.
__________________
Blog: chriskafer.wordpress.com Ignorance more frequently begets knowledge: it is those who know little, and not those who know much, who so positively assert that this or that problem will never be solved by science. --Charles Darwin |
July 26, 2013 | #24 | |
Tomatovillian™
Join Date: Nov 2008
Location: Minnesota
Posts: 568
|
Quote:
|
|
July 26, 2013 | #25 | |
Tomatovillian™
Join Date: Mar 2011
Location: Raleigh, NC
Posts: 1,448
|
Correct. Just wanted to be clear (or maybe just overly pedantic)
Quote:
__________________
Blog: chriskafer.wordpress.com Ignorance more frequently begets knowledge: it is those who know little, and not those who know much, who so positively assert that this or that problem will never be solved by science. --Charles Darwin |
|
|
|