Tomatoville® Gardening Forums


Notices

Forum area for discussing hybridizing tomatoes in technical terms and information pertinent to trait/variety specific long-term (1+ years) growout projects.

Reply
 
Thread Tools Display Modes
Old December 25, 2010   #151
frogsleap farm
Tomatovillian™
 
frogsleap farm's Avatar
 
Join Date: Nov 2008
Location: Minnesota
Posts: 568
Default

Virtually all my selected F2 plants were indeterminate. I had a couple of PL plants, as expected, when I crossed to a PL parent in the F1. I planted my selected F2 plants a little too close together and the plants were very vigorous and leafy. Fruit set in the canopy didn't get enough light for much anthocyanin accumulation, but fruit near the top and on the edges got plenty of light and did express the Aft trait.
frogsleap farm is offline   Reply With Quote
Old December 26, 2010   #152
Fred Hempel
Tomatovillian™
 
Fred Hempel's Avatar
 
Join Date: Dec 2010
Location: Sunol, CA
Posts: 2,723
Default

Do other factors like elevated temperature seem to work with light to promote anthocyanins?
Fred Hempel is offline   Reply With Quote
Old December 26, 2010   #153
frogsleap farm
Tomatovillian™
 
frogsleap farm's Avatar
 
Join Date: Nov 2008
Location: Minnesota
Posts: 568
Default

Quote:
Originally Posted by Fred Hempel View Post
Do other factors like elevated temperature seem to work with light to promote anthocyanins?
Temperature seems to have an effect, but its cool temperatures that stimulate anthocyanin accumulation. This is especially true in vegetative tissue.
frogsleap farm is offline   Reply With Quote
Old December 26, 2010   #154
casino
Tomatovillian™
 
casino's Avatar
 
Join Date: Mar 2010
Location: Southfield, Michigan
Posts: 318
Default

Nice looking tomato, to me thats a winner color combination. How does it taste?
casino is offline   Reply With Quote
Old March 6, 2011   #155
frogsleap farm
Tomatovillian™
 
frogsleap farm's Avatar
 
Join Date: Nov 2008
Location: Minnesota
Posts: 568
Default

Yesterday I planted the seed of F1, F2 and F3 lines from which we will select plants for our 2011 “Blue tomato” breeding nursery. In preparation for this I reviewed notes from our 2010 F2 nursery, a summary of which (w/ photos) I posted this morning at our tomato breeding blog @ www.frogsleapfarm.blogspot.com. We’ll also be including a couple of Tom Wagner’s new OSU Blue-derived lines in the new nursery this year.

Last edited by frogsleap farm; March 6, 2011 at 11:03 AM. Reason: typo
frogsleap farm is offline   Reply With Quote
Old March 6, 2011   #156
Fusion_power
Tomatovillian™
 
Fusion_power's Avatar
 
Join Date: Feb 2006
Location: Alabama
Posts: 2,250
Default

Frog, atv and abg are up-regulated in cool temps. aft is up-regulated at higher UV exposure. Because higher UV levels usually correspond with higher temps, this gives the impression that high temps are the cause of excess fruit anthocyanin when in fact the culprit is UV. atv tends to express in vegetative tissue while aft tends to express in the skin and pericarp of fruit.

I've got a couple of lines with seed started. One is a black on black from crossing P20 with a black fruited line. Another is a very sweet line derived from Sungold, Little Lucky, Lucky Cross, Purple Haze, and P20. The fruit are an almost iridescent brilliant red with dark blue/black shoulders.

DarJones
Fusion_power is offline   Reply With Quote
Old March 6, 2011   #157
frogsleap farm
Tomatovillian™
 
frogsleap farm's Avatar
 
Join Date: Nov 2008
Location: Minnesota
Posts: 568
Default

DarJones - I have no way of tracking abg, so not sure if it is even in play in my material, but your other comments are consistent with my experience. One of my striped Aft lines is a black on brown, and I agree that the brilliant red/blue-black contrast is stunning.
frogsleap farm is offline   Reply With Quote
Old March 16, 2011   #158
frogsleap farm
Tomatovillian™
 
frogsleap farm's Avatar
 
Join Date: Nov 2008
Location: Minnesota
Posts: 568
Default

First seeded, first up - F3 lines tracing to crosses with OSU Blue. F2 plants were selected for both strong anthocyanin expression in seedlings and in fruit. Great uniform expression of anthocyanin in hypocotyls of these blue/indigo lines.
Attached Images
File Type: jpg IMG_1705.jpg (125.4 KB, 91 views)
File Type: jpg IMG_1685.JPG (247.2 KB, 85 views)
frogsleap farm is offline   Reply With Quote
Old March 17, 2011   #159
dustdevil
Tomatovillian™
 
Join Date: Jan 2010
Location: WI, USA Zone4
Posts: 1,887
Default

The anthocyanin expression is very strong for such young seedlings. Does extra light bring out more "color" in the seedlings?
dustdevil is offline   Reply With Quote
Old March 17, 2011   #160
frogsleap farm
Tomatovillian™
 
frogsleap farm's Avatar
 
Join Date: Nov 2008
Location: Minnesota
Posts: 568
Default

Quote:
Originally Posted by dustdevil View Post
The anthocyanin expression is very strong for such young seedlings. Does extra light bring out more "color" in the seedlings?
Yes bright light helps, but you can see in the photo the very dark purple hypocotyl right at emergence. It was warm enough to move these outside for a while today, last year this really helped sort things out. However these F3 lines look very uniform for purple hypocoyls - atv and Aft may both be homozygous frm the intense selection last generation
frogsleap farm is offline   Reply With Quote
Old March 20, 2011   #161
frogsleap farm
Tomatovillian™
 
frogsleap farm's Avatar
 
Join Date: Nov 2008
Location: Minnesota
Posts: 568
Default

I put these seedlings under a UV light this afternoon. Generally anthocyanin production in plants is up-regulated by UV light, which was lacking with my indoor set up. It's too cool for the outdoor natural light treatment, so I decided to try UV supplementation to sort out the progeny.
frogsleap farm is offline   Reply With Quote
Old March 20, 2011   #162
Fusion_power
Tomatovillian™
 
Fusion_power's Avatar
 
Join Date: Feb 2006
Location: Alabama
Posts: 2,250
Default

Frog, atv is expressed in vegetative tissue. When you select for deep purple seedlings, you are selecting for homozygous atv. The only way to consistently select for homozygous aft is from expression in fruit exposed to direct sunlight.

DarJones
Fusion_power is offline   Reply With Quote
Old March 21, 2011   #163
frogsleap farm
Tomatovillian™
 
frogsleap farm's Avatar
 
Join Date: Nov 2008
Location: Minnesota
Posts: 568
Default

Yep, I'm at the seedling stage now and selecting for atv. The UV treatment is designed to help in that selection. It will unfortunately be a while before I can get these seedlings significantly exposed to outdoor natural light, and I have to winnow numbers down soon.

Quote:
Originally Posted by Fusion_power View Post
Frog, atv is expressed in vegetative tissue. When you select for deep purple seedlings, you are selecting for homozygous atv. The only way to consistently select for homozygous aft is from expression in fruit exposed to direct sunlight.

DarJones
frogsleap farm is offline   Reply With Quote
Old March 27, 2011   #164
frogsleap farm
Tomatovillian™
 
frogsleap farm's Avatar
 
Join Date: Nov 2008
Location: Minnesota
Posts: 568
Default

Seedling selections for anthocyanin done and transplants recovering well. Here are two F3 lines, one large fruited and segregating for PL, the other a red cherry.
Attached Images
File Type: jpg IMG_1731.jpg (252.3 KB, 64 views)
File Type: jpg IMG_1736.JPG (438.5 KB, 57 views)
frogsleap farm is offline   Reply With Quote
Old March 28, 2011   #165
dustdevil
Tomatovillian™
 
Join Date: Jan 2010
Location: WI, USA Zone4
Posts: 1,887
Default

Hi Frog.

I enjoy your tomato breeding posts here and on your site. The photos are nice!

Dust
dustdevil is offline   Reply With Quote
Reply

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

Forum Jump


All times are GMT -4. The time now is 05:05 AM.


★ Tomatoville® is a registered trademark of Commerce Holdings, LLC ★ All Content ©2022 Commerce Holdings, LLC ★