I collect the pollen with an electric toothbrush and a small square of
plastic wrap. The plastic wrap lacks any rigidity that could damage
the pistil when coating the tip of it with pollen (although watch out for
the wind if doing this outdoors), and you can see fresh pollen on there
easily enough.
I trim flowers just before they open, like if I waited another couple of
days it would be too late, those buds would open. Sometimes I get
self-pollenated seeds that were pollenated before the bud ever opened,
but with an RL male pollen donor and a PL female bud, that is easy
to see in the F1 seedlings (if the F1 is RL, the cross was successful).
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