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Old March 21, 2014   #1
bower
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Join Date: Feb 2012
Location: Newfoundland, Canada
Posts: 6,793
Default advice on breeding with lycopersicoides introgression lines

Looking for advice from Dar Jones and/or others about whether it's better to use the lycopersicoides IL's as pollen donor or female parent, or if it makes no difference.

The TGRC method of 1/2 hour soak in 1/2 strength bleach worked really well, so a couple of days later I have three healthy seedlings that successfully shucked their seed coats, of the botrytis resistant LA 4268 which Dar sent me last year (thank you!!). Very excited about the chance to breed some resistant lines and make some crosses early in the season this time.

I have limited space for the mother plants in the greenhouse, and want to ensure they get the best positions I can offer. If there is no difficulty using LA4268 as the mother though, I could easily start some extra varieties to use as pollen donors, and let them take their chances in smaller containers and outdoors, once they've set a few fruit for self seed in the pollinator-free zone.

So to rephrase the question, to Dar and others who have done some breeding work with the lycopersicoides IL's in VF36 background, is the receptivity as a female parent average cw tomatoes or more prone to fail? Or equivalent to success rates as pollen donor.

I haven't yet tried the LA 4233 line, btw, due to space constraints and because TGRC described as "highly sterile", which makes me think it needs more expert breeding techniques than I have presently in my arsenal as a rank amateur.
TIA for all advice!
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